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EYS/RP25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90038

Novus Biologicals, part of Bio-Techne
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NBP1-90038
NBP1-90038-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CECTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCR

Reactivity Notes

Zebrafish reactivity was listed previously based on publications citing use with previous lot (A104781). Current lot has been reported by customers to not be reactive with Zebrafish.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYS/RP25 Antibody

Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038]

Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038]

Immunocytochemistry/Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] - Wild-type & pomgnt1sny7 mutant retinas: EYS/RP25 (green) & acetylated alpha-tubulin (red). (A,E) Wild-type retina 2-mpf. (C,G) Wild-type retina 6-mpf. (B, F) Homozygous pomgnt1sny7 mutant retina 2-mpf. (D,H) Homozygous pomgnt1sny7 mutant retina 6-mpf. (I,J) Counting EYS-acetylated alpha-tubulin from outer nuclear layer to retinal pigment epithelium (RPE) 2-mpf and 6-mpf retina. (K,L) Ratio of EYS/RP25 & acetylated alpha-tubulin-labeled axonemes in rods & cones 2-mpf (M,N). Ratio of EYS/RP25 and acetylated alpha-tubulin acetylated alpha-tubulin-labeled axoneme in rods & cones 6-mpf (O,Q). Total EYS/RP25 immunoreactivity 2-mpf & 6-mpf. (P,R) Total EYS/RP25 in wild-type/heterozygous & mutant fish 2-mpf, 6-mpf. Scale bar: 5 um (A-D); 2.5 um (E-H). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-020-64752-4), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: EYS/RP25 Antibody [NBP1-90038]

Immunohistochemistry-Paraffin: EYS/RP25 Antibody [NBP1-90038]

Immunohistochemistry-Paraffin: EYS/RP25 Antibody [NBP1-90038] - Staining of human lung shows moderate cytoplasmic positivity in lung macrophages.
EYS/RP25 Antibody

Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] -

Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] - Immunohistochemistry on retinal sections of wild-type & eysrmc101/rmc101 zebrafish.(A) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf & 5 mpf stained with antibodies against Eys (green) & centrin (red). (B) BODIPY (green) staining showing disorganization of photoreceptor outer segments in eysrmc101/rmc101 zebrafish (5 dpf & 5 mpf) compared to age- & strain-matched wild-type zebrafish (arrows). (C) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf (upper panel), 2 mpf (middle panel) & 5 mpf (lower panel) stained with antibodies against rhodopsin (green) & F-actin (red). Asterisks indicate mislocalization of rhodopsin to the inner segments & synapses of photoreceptor cells. (D) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf, 2 mpf & 5 mpf stained with antibodies against GNAT2 (green) & F-actin (red). Arrows indicate dysmorphic outer segments in mutant zebrafish. In all images, nuclei are counterstained with DAPI (blue). INL: inner nuclear layer; ONL: outer nuclear layer; OS: outer segments. Scale: 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30052645), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for EYS/RP25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 27737822).

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

Reported in the scientific literature (PMID: 30052645).

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EYS/RP25

LOC442228 - similar to dJ22I17.2 (novel protein with EGF-like and laminin G domains)

Alternate Names

C6orf178, C6orf179, C6orf180, dJ22I17.2, dJ303F19.1, EGFL10, EGFL11, EGF-like-domain, multiple 10, EGF-like-domain, multiple 11, eyes shut homolog (Drosophila), protein spacemaker homolog

Gene Symbol

EYS

Additional EYS/RP25 Products

Product Documents for EYS/RP25 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EYS/RP25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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