EYS/RP25 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90038
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Fish - Danio rerio (Zebrafish)
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CECTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCR
Reactivity Notes
Zebrafish reactivity was listed previously based on publications citing use with previous lot (A104781). Current lot has been reported by customers to not be reactive with Zebrafish.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EYS/RP25 Antibody
Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038]
Immunocytochemistry/Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] - Wild-type & pomgnt1sny7 mutant retinas: EYS/RP25 (green) & acetylated alpha-tubulin (red). (A,E) Wild-type retina 2-mpf. (C,G) Wild-type retina 6-mpf. (B, F) Homozygous pomgnt1sny7 mutant retina 2-mpf. (D,H) Homozygous pomgnt1sny7 mutant retina 6-mpf. (I,J) Counting EYS-acetylated alpha-tubulin from outer nuclear layer to retinal pigment epithelium (RPE) 2-mpf and 6-mpf retina. (K,L) Ratio of EYS/RP25 & acetylated alpha-tubulin-labeled axonemes in rods & cones 2-mpf (M,N). Ratio of EYS/RP25 and acetylated alpha-tubulin acetylated alpha-tubulin-labeled axoneme in rods & cones 6-mpf (O,Q). Total EYS/RP25 immunoreactivity 2-mpf & 6-mpf. (P,R) Total EYS/RP25 in wild-type/heterozygous & mutant fish 2-mpf, 6-mpf. Scale bar: 5 um (A-D); 2.5 um (E-H). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-020-64752-4), licensed under a CC-BY license.Immunohistochemistry-Paraffin: EYS/RP25 Antibody [NBP1-90038]
Immunohistochemistry-Paraffin: EYS/RP25 Antibody [NBP1-90038] - Staining of human lung shows moderate cytoplasmic positivity in lung macrophages.Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] -
Immunocytochemistry/ Immunofluorescence: EYS/RP25 Antibody [NBP1-90038] - Immunohistochemistry on retinal sections of wild-type & eysrmc101/rmc101 zebrafish.(A) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf & 5 mpf stained with antibodies against Eys (green) & centrin (red). (B) BODIPY (green) staining showing disorganization of photoreceptor outer segments in eysrmc101/rmc101 zebrafish (5 dpf & 5 mpf) compared to age- & strain-matched wild-type zebrafish (arrows). (C) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf (upper panel), 2 mpf (middle panel) & 5 mpf (lower panel) stained with antibodies against rhodopsin (green) & F-actin (red). Asterisks indicate mislocalization of rhodopsin to the inner segments & synapses of photoreceptor cells. (D) Retinal sections of wild-type & eysrmc101/rmc101 zebrafish at 5 dpf, 2 mpf & 5 mpf stained with antibodies against GNAT2 (green) & F-actin (red). Arrows indicate dysmorphic outer segments in mutant zebrafish. In all images, nuclei are counterstained with DAPI (blue). INL: inner nuclear layer; ONL: outer nuclear layer; OS: outer segments. Scale: 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30052645), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for EYS/RP25 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID 27737822).
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Frozen
Reported in the scientific literature (PMID: 30052645).
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EYS/RP25
Alternate Names
C6orf178, C6orf179, C6orf180, dJ22I17.2, dJ303F19.1, EGFL10, EGFL11, EGF-like-domain, multiple 10, EGF-like-domain, multiple 11, eyes shut homolog (Drosophila), protein spacemaker homolog
Gene Symbol
EYS
Additional EYS/RP25 Products
Product Documents for EYS/RP25 Antibody
Product Specific Notices for EYS/RP25 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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