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EZH2/KMT6 Antibody (9Q2R9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15783

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15783-100ul
NBP3-15783-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q2R9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for EZH2/KMT6 Antibody (9Q2R9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 148-272 of human EZH2/KMT6 (Q15910). ELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783]

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783]

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783] - Immunohistochemistry of paraffin-embedded mouse spleen using EZH2/KMT6 antibody (NBP3-15783) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
EZH2/KMT6 Antibody (9Q2R9)

Western Blot: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783] -

Western Blot: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783] - analysis of extracts of various cell lines, using EZH2/KMT6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.

Applications for EZH2/KMT6 Antibody (9Q2R9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EZH2

KMT6 / EZH2 encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Enhancer of Zeste Homolog 2 [Drosophila]

Alternate Names

ENX1, KMT6

Gene Symbol

EZH2

Additional EZH2 Products

Product Documents for EZH2/KMT6 Antibody (9Q2R9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EZH2/KMT6 Antibody (9Q2R9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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