EZH2/KMT6 Antibody (CL11915)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21204

Novus Biologicals, part of Bio-Techne
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NBP3-21204-100ul
NBP3-21204-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL11915

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EZH2 Antibodies »

Product Summary for EZH2/KMT6 Antibody (CL11915)

Immunogen

This antibody was developed against a Synthetic Peptide of Q15910. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for EZH2/KMT6 Antibody (CL11915)

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody [NBP3-21204] -

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody [NBP3-21204] -

Analysis in human testis and cerebral cortex tissues using NBP3-21204 antibody. Corresponding EZH2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: EZH2/KMT6 Antibody [NBP3-21204] -

Immunocytochemistry/Immunofluorescence: EZH2/KMT6 Antibody [NBP3-21204] -

Staining of U-251 cells using the Anti-EZH2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.
Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody [NBP3-21204] -

Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody [NBP3-21204] -

Staining of human kidney shows mainly absence of positivity in cells in tubules as expected.
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Applications for EZH2/KMT6 Antibody (CL11915)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EZH2

KMT6 / EZH2 encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Enhancer of Zeste Homolog 2 [Drosophila]

Alternate Names

ENX1, KMT6

Gene Symbol

EZH2

Additional EZH2 Products

Product Documents for EZH2/KMT6 Antibody (CL11915)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EZH2/KMT6 Antibody (CL11915)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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