EZH2/KMT6 Antibody (EZH2/6988)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20652
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Kappa Clone # EZH2/6988
Concentration
0.2 mg/ml
Product Summary for EZH2/KMT6 Antibody (EZH2/6988)
Immunogen
Recombinant fragment (around aa300-550) of human EZH2/KMT6 protein (exact sequence is proprietary)
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG Kappa
Description
Positive Controls: MCF7, HeLa, U-2 OS or HEK-293 whole cell lysates. Human tonsil, testis or prostate tissue.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for EZH2/KMT6 Antibody (EZH2/6988)
Western Blot Analysis of Jurkat cell lysate using EZH2/KMT6 antibody (EZH2/6988).
EZH2/KMT6 Antibody (EZH2/6988)
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with EZH2/KMT6 antibody (EZH2/6988). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
EZH2/KMT6 Antibody (EZH2/6988)
FFPE human Lynch syndrome colon carcinoma stained with EZH2/KMT6 antibody (EZH2/6988). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for EZH2/KMT6 Antibody (EZH2/6988)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EZH2
Long Name
Enhancer of Zeste Homolog 2 [Drosophila]
Alternate Names
ENX1, KMT6
Gene Symbol
EZH2
Additional EZH2 Products
Product Documents for EZH2/KMT6 Antibody (EZH2/6988)
Product Specific Notices for EZH2/KMT6 Antibody (EZH2/6988)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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