EZH2/KMT6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17087
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (99%), Fish (95%), Mouse (99%), Porcine (99%), Rhesus Macaque (100%), Xenopus (97%), Zebrafish (94%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human EZH2/KMT6. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (97%), Japanese medaka (95%),
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EZH2/KMT6 Antibody
![Western Blot: EZH2/KMT6 Antibody [NBP2-17087]](https://resources.bio-techne.com/images/products/KMT6-EZH2-Antibody-Western-Blot-NBP2-17087-img0001.jpg "Western Blot: EZH2/KMT6 Antibody [NBP2-17087]")
Western Blot: EZH2/KMT6 Antibody [NBP2-17087]
Western Blot: KMT6/EZH2 Antibody [NBP2-17087] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 C: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087]](https://resources.bio-techne.com/images/products/EZH2-KMT6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17087-img0002.jpg "Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087]")
Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087]
Immunocytochemistry/Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EZH2 protein stained by EZH2 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Chromatin Immunoprecipitation: EZH2/KMT6 Antibody [NBP2-17087] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
Applications for EZH2/KMT6 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EZH2
Long Name
Enhancer of Zeste Homolog 2 [Drosophila]
Alternate Names
ENX1, KMT6
Gene Symbol
EZH2
UniProt
Additional EZH2 Products
Product Documents for EZH2/KMT6 Antibody
Product Specific Notices for EZH2/KMT6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.