Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16959
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around T567 of human Ezrin/Radixin/Moesin (NP_003370.2). YKTLR
Modification
p Thr 567, p Thr 564, p Thr 558
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
![Western Blot: Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] AntibodyAzide and BSA Free [NBP3-16959]](https://resources.bio-techne.com/images/products/Ezrin-Radixin-Moesin-[p-Thr567-p-Thr564-p-Thr558]-Antibody-Western-Blot-NBP3-16959-img0001.jpg "Western Blot: Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] AntibodyAzide and BSA Free [NBP3-16959]")
Western Blot: Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] AntibodyAzide and BSA Free [NBP3-16959]
Western Blot: Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody [NBP3-16959] - Western blot analysis of extracts of various cell lines, using Ezrin/Radixin/Moesin antibody (NBP3-16959) at 1:1000 dilution.HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Applications
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Application Notes
Modifications: Ezrin - p Thr 567, Radixin - p Thr 564, Moesin - p Thr 558
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ezrin/Radixin/Moesin
Alternate Names
CVIL, CVL, Cytovillin, DFNB24, EZR, HEL70, HEL-S-105, IMD50, Membrane-organizing extension spike protein, MSN, p81, RDX, villin-2
Gene Symbol
EZR
Additional Ezrin/Radixin/Moesin Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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