F-Spondin/SPON1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69044
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for F-Spondin/SPON1 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for F-Spondin/SPON1 Antibody
Western Blot Shows Human F-Spondin/SPON1 Specificity Using Knockout Cell Line.
Western blot shows lysates of OVCAR3 and F-Spondin/SPON1 knockout OVCAR3 cell line (KO). Nitrocellulose membrane was probed with F-Spondin/SPON1 Antibody (Catalog # NBP2-69044) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for F-Spondin/SPON1 (as indicated) in the parental OVCAR3 cell line, but is not detectable in knockout OVCAR3 cell line. Antibody dilution used: 1/500. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).![Immunohistochemistry-Paraffin: F-Spondin/SPON1 Antibody [NBP2-69044]](https://resources.bio-techne.com/images/products/F-Spondin-SPON1-Antibody-Immunohistochemistry-NBP2-69044-img0001.jpg "Immunohistochemistry-Paraffin: F-Spondin/SPON1 Antibody [NBP2-69044]")
Immunohistochemistry-Paraffin: F-Spondin/SPON1 Antibody [NBP2-69044]
Immunohistochemistry-Paraffin: F-Spondin/SPON1 Antibody [NBP2-69044] - Staining of human small intestine shows positivity in glandular cells, smooth muscle and plasma.Applications for F-Spondin/SPON1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Knockout Validated
Application from YCharOS.
Western Blot
Application from YCharOS.
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: F-Spondin/SPON1
Alternate Names
FSpondin, SPON1, VSGP
Gene Symbol
SPON1
Additional F-Spondin/SPON1 Products
Product Documents for F-Spondin/SPON1 Antibody
Product Specific Notices for F-Spondin/SPON1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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