F4/80 Antibody (Cl:A3-1 (recombinant version)) [Janelia Fluor® 669]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81029JF669
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Label
Janelia Fluor 669
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Cl:A3-1 (recombinant version)
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for F4/80 Antibody (Cl:A3-1 (recombinant version)) [Janelia Fluor® 669]
Immunogen
This F4/80 Antibody (Cl:A3-1 (recombinant version)) - Chimeric was developed against Thioglycollate stimulated peritoneal macrophages of mouse origin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Applications for F4/80 Antibody (Cl:A3-1 (recombinant version)) [Janelia Fluor® 669]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: F4/80
Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).
References
1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7
2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15
Alternate Names
DD7A5-7, Gpf480, Ly71
Gene Symbol
Adgre1
Additional F4/80 Products
Product Specific Notices for F4/80 Antibody (Cl:A3-1 (recombinant version)) [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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