FACL4 Antibody (4W3T9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16012

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16012-100ul
NBP3-16012-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4W3T9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FACL4 Antibodies »

Product Summary for FACL4 Antibody (4W3T9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human FACL4 (NP_075266.1). MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FACL4 Antibody (4W3T9)

Western Blot: FACL4 Antibody (4W3T9) [NBP3-16012]

Western Blot: FACL4 Antibody (4W3T9) [NBP3-16012]

Western Blot: FACL4 Antibody (3I8D10) [NBP3-16012] - Western blot analysis of extracts of various cell lines, using FACL4 antibody (NBP3-16012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: FACL4 Antibody (4W3T9) [NBP3-16012]

Immunoprecipitation: FACL4 Antibody (4W3T9) [NBP3-16012]

Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.

Applications for FACL4 Antibody (4W3T9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:10000 - 1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Gene Symbol

ACSL4

Additional FACL4 Products

Product Documents for FACL4 Antibody (4W3T9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FACL4 Antibody (4W3T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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