FACL4 Antibody (HL229)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13666
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Drosophila
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL229
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FACL4 Antibody (HL229)
Western Blot: FACL4 Antibody (HL229) [NBP3-13666]
Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666]
Immunocytochemistry/Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666]
Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for FACL4 Antibody (HL229)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FACL4
Alternate Names
ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63
Gene Symbol
ACSL4
Additional FACL4 Products
Product Documents for FACL4 Antibody (HL229)
Product Specific Notices for FACL4 Antibody (HL229)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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