Factor XII Antibody (10-11-37)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11299

Novus Biologicals, part of Bio-Techne
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NBP3-11299
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 10-11-37

Concentration

0.1 mg/ml

View all Factor XII Antibodies »

Product Specifications

Immunogen

Factor XII adsorbed onto Al(OH)3 adjuvant and emulsified in Freund's Incomplete Adjuvant

Specificity

The antibody is specific for the heavy chain of Factor XIIa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Factor XII Antibody (10-11-37)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Factor XII

Monoclonal antibody 10-11-37 recognizes Coagulation Factor XII (FXII, Hageman factor). FXII is a serine protease and plays a role in blood coagulation, fibrinolysis, kinin and complement systems. The protein is the zymogen of the serine protease factor XIIa (FXIIa). FXII is converted to FXIIa through autoactivation induced by contact to charged surfaces, also known as the plasma contact system. FXII is predominantly synthesized in the liver and is composed of fibronectin type I and II domains, two epidermal growth factor-like domains, a kringle region, a proline-rich domain and a catalytic domain. Its molecular weight is approximately 80kDa on SDS-PAGE gel electroforeses. The protein circulates in the plasma at a concentration of 30-35 ug/ml. FXII forms the plasma contact system together with high molecular weight kininogen and plasma kalikrein. FXII autoactivates when these three proteins form a complex on negatively charged nonphysiological surfaces, like inorganic surfaces (eg silicon tubes) or macromolecular organic surfaces (eg heparin) bound to the surface of different cell types, including endothelial cells, platelets and neutrophils. It can trigger blood coagulation and generation of proinflammatory bradykinin. After surface complexation, FXII autoactivates into FXIIa, also called factor XII fragment(XIIf). Once small amounts of kalikrein are formed a positive feedback loop is active leading to enhanced conversion into FXIIa. The activation leads to a series of active enzyme formation. FXIIa converts prekallikrein to kallikrein and kallikrein digests kinogen to liberate proinflammatory bradykinin. Bradykinin triggers inflammatory reactions via activating endothelial cells resulting in vasodilatation, increased vascular permeability and production of other mediators like nitric oxide. The contact system has the ability to activate the complement system via the classical pathway. Simultaneous activation of both systems may lead to pathological conditions, like hereditary angioedema in individuals with dysfunctional C1-inhibitor (C1-IHB). FXIIa can activate complement protein C1r and to a lesser degree C1s in absence of C1-IHB. This leads to unimpeded bradykinin formation resulting in angioedema. Other interactions with complement system are found on the level of gC1qR and MASP-1. The antibody is specific for the heavy chain of FXIIa.

Alternate Names

beta-factor XIIa part 1, coagulation factor XII, coagulation factor XII (Hageman factor), coagulation factor XIIa heavy chain, coagulation factor XIIa light chain, EC 3.4.21, EC 3.4.21.38, F12, HAE3, HAEX, HAF, HAF beta-factor XIIa part 2, Hageman factor

Gene Symbol

F12

Additional Factor XII Products

Product Documents for Factor XII Antibody (10-11-37)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Factor XII Antibody (10-11-37)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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