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FAM185A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93478

Novus Biologicals, part of Bio-Techne
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NBP1-93478
NBP1-93478-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RTLKEWTLQVSPFGRLRARLPCHLAVRPLDPLTYPDGDRVLVAVCGVEGGVRGLDGLQVKYDEDLEEMAIVSDTIHPQASVEV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAM185A Antibody

Western Blot: FAM185A Antibody [NBP1-93478]

Western Blot: FAM185A Antibody [NBP1-93478]

Western Blot: FAM185A Antibody [NBP1-93478] - Analysis in control (vector only transfected HEK293T lysate) and FAM185A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FAM185A Antibody [NBP1-93478]

Immunocytochemistry/ Immunofluorescence: FAM185A Antibody [NBP1-93478]

Immunocytochemistry/Immunofluorescence: FAM185A Antibody [NBP1-93478] - Staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: FAM185A Antibody [NBP1-93478]

Immunohistochemistry-Paraffin: FAM185A Antibody [NBP1-93478]

Immunohistochemistry-Paraffin: FAM185A Antibody [NBP1-93478] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for FAM185A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAM185A

Alternate Names

family with sequence similarity 185, member A, hypothetical protein LOC222234

Gene Symbol

FAM185A

Additional FAM185A Products

Product Documents for FAM185A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FAM185A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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