FAM50A Antibody (JE65-21)

Western Blot: FAM50A Antibody (JE65-21) [NBP3-32332]

Western Blot: FAM50A Antibody (JE65-21) [NBP3-32332] - Western blot analysis of FAM50A on different lysates with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/1,000 dilution.

Lane 1: U87MG cell lysate
Lane 2: U937 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32332) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: FAM50A Antibody (JE65-21) [NBP3-32332]

Immunohistochemistry: FAM50A Antibody (JE65-21) [NBP3-32332] - Immunohistochemical analysis of paraffin-embedded rat rectum tissue with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32332) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: FAM50A Antibody (JE65-21) [NBP3-32332]

Immunocytochemistry/ Immunofluorescence: FAM50A Antibody (JE65-21) [NBP3-32332] - Immunocytochemistry analysis of NCI-H441 cells labeling FAM50A with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAM50A antibody (NBP3-32332) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.