FAM50A Antibody

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344]

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human small intestine shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344]

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human cerebral cortex strong nuclear/cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344]

Immunohistochemistry-Paraffin: FAM50A Antibody [NBP1-89344] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Western Blot: FAM50A Antibody [NBP1-89344] -

Western Blot: FAM50A Antibody [NBP1-89344] - FAM50A interacts with U5 & C-complex proteins.a Schematic representation of the two-step splicing reaction (adapted from ref. 70). EFTUD2 (U5); FAM50A & DDX41 (C-complex). b Graphical representation of semi-native co-immunoprecipitation assay. Candidate interactors were overexpressed in U-87 cells, harvested in immunoprecipitation (IP) lysis buffer at 60 h post-transfection & immunoprecipitated with anti-FAM50A antibody. The interaction partners were detected in IP lysate using anti-V5 & anti-FAM50A antibodies, respectively. c, d Western blot of proteins after co-immunoprecipitation. Top: total protein lysate input (50 μg/lane) was migrated on 4–15% polyacrylamide gels to detect EFTUD2 (c) or DDX41 (d) using anti-V5 mouse monoclonal antibody. GAPDH was used as loading control. Bottom: Anti-FAM50A antibody was used to immunoprecipitate native FAM50A protein in total input lysate of 2.3 mg/condition (c) or 3.5 mg/condition (d). The IP lysate was separated in two parts (20% & 80%) & migrated independently on 4–15% polyacrylamide gels. The proteins of interest with predicated band sizes are indicated with black arrows. Plus & minus signs indicate presence & absence of relevant plasmids, respectively. EFTUD2 & DDX41 were detected in independent experiments using protein lysates derived from replicate batches of U-87 cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32703943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.