Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all FAM8A1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human FAM8A1 protein (between amino acids 50-100) [Uniprot Q9UBU6]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAM8A1 Antibody - BSA Free

Western Blot: FAM8A1 AntibodyBSA Free [NBP2-47125]

Western Blot: FAM8A1 AntibodyBSA Free [NBP2-47125]

Western Blot: FAM8A1 Antibody [NBP2-47125] - Western Blot Image of anti-FAM8A1. Total protein from human brain (lane 1), skeletal muscle (lane 2), mouse brain (lane 3) and rat testis (lane 4) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM8A1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FAM8A1 Antibody - BSA Free [NBP2-47125]

Immunocytochemistry/ Immunofluorescence: FAM8A1 Antibody - BSA Free [NBP2-47125]

Immunocytochemistry/Immunofluorescence: FAM8A1 Antibody [NBP2-47125] - Hek293 cells were fixed for 5 minute using 10% formalin and then permeabilized for 10 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-FAM8A1 at a 1:500 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: FAM8A1 Antibody - BSA Free [NBP2-47125]

Immunohistochemistry-Paraffin: FAM8A1 Antibody - BSA Free [NBP2-47125]

Immunohistochemistry-Paraffin: FAM8A1 Antibody [NBP2-47125] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse testes using FAM8A1 antibody at 1:500 dilution. The antibody generated a cytoplasmic staining with intense brown dots (potentially the staining of the ER) in each of the primary and secondary spermatocytic cells.
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Applications for FAM8A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1.0 - 2.0 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAM8A1

Alternate Names

AHCP

Gene Symbol

FAM8A1

UniProt

Q9UBU6 (Human)

Additional FAM8A1 Products

Product Documents for FAM8A1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FAM8A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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