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FARS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-57378

Novus Biologicals, part of Bio-Techne
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NBP1-57378

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to FARS2(phenylalanyl-tRNA synthetase 2, mitochondrial) The peptide sequence was selected from the N terminal of FARS2. Peptide sequence VELLGKSYPQDDHSNLTRKVLTRVGRNLHNQQHHPLWLIKERVKEHFYKQ. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for FARS2 Antibody

Western Blot: FARS2 Antibody [NBP1-57378]

Western Blot: FARS2 Antibody [NBP1-57378]

Western Blot: FARS2 Antibody [NBP1-57378] - Transfected 293T, Antibody Titration: 5.0ug/ml
Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-57378]

Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-57378]

Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-57378] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.

Applications for FARS2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FARS2

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm.Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.

Alternate Names

dJ236A3.1, dJ520B18.2, dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase)), EC 6.1.1, EC 6.1.1.20, FARS1dJ236A3.1 (phenylalanine-tRNA synthetase), HSPC320, phenylalanine translase, phenylalanine tRNA ligase 2, mitochondrial, Phenylalanine--tRNA ligase, phenylalanine-tRNA synthetase 1 (mitochondrial), phenylalanine-tRNA synthetase 2 (mitochondrial), phenylalanyl-tRNA synthetase 2, mitochondrial, phenylalanyl-tRNA synthetase, mitochondrial, PheRS

Gene Symbol

FARS2

UniProt

Additional FARS2 Products

Product Documents for FARS2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FARS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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