Fascin Antibody (1B3V6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16828

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16828-100ul
NBP3-16828-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1B3V6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fascin Antibodies »

Product Summary for Fascin Antibody (1B3V6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 249-362 of human Fascin (Q16658). GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fascin Antibody (1B3V6)

Western Blot: Fascin Antibody (1B3V6) [NBP3-16828]

Western Blot: Fascin Antibody (1B3V6) [NBP3-16828]

Western Blot: Fascin Antibody (1B3V6) [NBP3-16828] - Western blot analysis of extracts of various cell lines, using Fascin Rabbit mAb (NBP3-16828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828]

Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828]

Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828] - Immunohistochemistry of paraffin-embedded mouse spleen using Fascin Rabbit mAb (NBP3-16828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828]

Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828]

Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828] - Immunohistochemistry of paraffin-embedded rat spleen using Fascin Rabbit mAb (NBP3-16828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Fascin Antibody (1B3V6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fascin

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Alternate Names

FAN1, FSCN1, HSN, Singed-like Protein, SNL

Gene Symbol

FSCN1

Additional Fascin Products

Product Documents for Fascin Antibody (1B3V6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fascin Antibody (1B3V6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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