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Fast skeletal myosin light chain 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16433

Novus Biologicals, part of Bio-Techne
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NBP2-16433

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fast skeletal myosin light chain 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fast skeletal myosin light chain 1 Antibody

Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - RD whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Fast skeletal myosin light chain 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Immunocytochemistry/ Immunofluorescence: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Immunocytochemistry/Immunofluorescence: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433]

Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Sample (whole cell lysate) A: 1ug mouse heart, B: 5ug mouse heart, 15% SDS PAGE gel, diluted at 1:10000.

Applications for Fast skeletal myosin light chain 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fast skeletal myosin light chain 1

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq]

Alternate Names

A1 catalytic, A2 catalytic, MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, myosin light chain 1/3, skeletal muscle isoform, Myosin light chain A1/A2, Myosin light chain alkali 1/2, myosin, light chain 1, alkali; skeletal, fast, myosin, light polypeptide 1, alkali; skeletal, fast

Gene Symbol

MYL1

UniProt

Additional Fast skeletal myosin light chain 1 Products

Product Documents for Fast skeletal myosin light chain 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fast skeletal myosin light chain 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.