Skip to main content

FAT1 Antibody (FAT1-3D7/1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07206

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-07206-100ug
NBP3-07206-20ug

Key Product Details

Species Reactivity

Drosophila

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # FAT1-3D7/1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Cytoplasmic domain of Drosophila Fat protein. (Uniprot: Q14517)

Reactivity Notes

Expected to cross-react with Chimpanzee. Rat (92%. 85% identity with immunogen. respectively) due to sequence homology.

Localization

Cell membrane. Nucleus. Cytoplasm > perinuclear region.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

500 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein L. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08951)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for FAT1 Antibody (FAT1-3D7/1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein L purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: FAT1

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Alternate Names

FAT tumor suppressor 1, FAT tumor suppressor homolog 1 (Drosophila)

Gene Symbol

FAT1

UniProt

Additional FAT1 Products

Product Documents for FAT1 Antibody (FAT1-3D7/1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FAT1 Antibody (FAT1-3D7/1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...