FAT10 Antibody (JJ084-09)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67917

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67917
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ084-09

Concentration

1 mg/ml

View all FAT10 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human FAT10 aa 114-158 / 165. (SwissProt: O15205 Human; SwissProt: P63072 Mouse)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT10 Antibody (JJ084-09)

Western Blot: FAT10 Antibody (JJ084-09) [NBP2-67917]

Western Blot: FAT10 Antibody (JJ084-09) [NBP2-67917]

Western Blot: FAT10 Antibody (JJ084-09) [NBP2-67917] - Analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917]

Immunocytochemistry/ Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917]

Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - Staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: FAT10 Antibody (JJ084-09) [NBP2-67917]

Immunohistochemistry-Paraffin: FAT10 Antibody (JJ084-09) [NBP2-67917]

Immunohistochemistry-Paraffin: FAT10 Antibody (JJ084-09) [NBP2-67917] - Analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.
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Applications for FAT10 Antibody (JJ084-09)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAT10

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10. Human FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, but more than one mRNA transcript has been identified for murine FAT10. FAT10 can also be induced by IFN-gamma and TNF-alpha in some cell lines. Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10-conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein. In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L. FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response.

Alternate Names

UBD

Gene Symbol

UBD

Additional FAT10 Products

Product Documents for FAT10 Antibody (JJ084-09)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FAT10 Antibody (JJ084-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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