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FBXL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1289

Novus Biologicals, part of Bio-Techne
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NB100-1289

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence VFSNNDEGLINKK-C corresponding to N-Terminus according to NP_036289.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FBXL2 Antibody

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NB100-1289]

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NB100-1289]

Immunocytochemistry/Immunofluorescence: FBXL2 Antibody [NB100-1289] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 8 ug/mL.

Applications for FBXL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

8 ug/mL

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:128000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXL2

FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.

Alternate Names

FBL2FBL3F-box protein containing leucine-rich repeats, F-box and leucine-rich repeat protein 2DKFZp564P0622, F-box protein FBL2/FBL3, F-box/LRR-repeat protein 2

Entrez Gene IDs

25827 (Human)

Gene Symbol

FBXL2

Additional FBXL2 Products

Product Documents for FBXL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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