FBXO3 Antibody (PSH04-09)

Western Blot: FBXO3 Antibody (PSH04-09) [NBP3-32334]

Western Blot: FBXO3 Antibody (PSH04-09) [NBP3-32334] - Western blot analysis of FBXO3 on different lysates with Rabbit anti-FBXO3 antibody (NBP3-32334) at 1/2,000 dilution.

Lane 1: MDA-MB-231 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: JAR cell lysate
Lane 7: HepG2 cell lysate
Lane 8: NIH/3T3 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 55 kDa
Observed band size: 55/48 kDa

Exposure time: 1 minute 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32334) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.