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FBXO31 Antibody (OTI1H1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01508

Novus Biologicals, part of Bio-Techne
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NBP2-01508
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H1

Concentration

0.85 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human FBXO31(NP_079011) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

60.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO31 Antibody (OTI1H1)

Western Blot: FBXO31 Antibody (OTI1H1) [NBP2-01508]

Western Blot: FBXO31 Antibody (OTI1H1) [NBP2-01508]

Western Blot: FBXO31 Antibody (1H1) [NBP2-01508] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO31 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO31.
Immunocytochemistry/Immunofluorescence: FBXO31 Antibody (1H1) [NBP2-01508] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO31.
Flow Cytometry: FBXO31 Antibody (OTI1H1) [NBP2-01508]

Flow Cytometry: FBXO31 Antibody (OTI1H1) [NBP2-01508]

Flow Cytometry: FBXO31 Antibody (1H1) [NBP2-01508] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO31 antibody, and then analyzed by flow cytometry.

Applications for FBXO31 Antibody (OTI1H1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.85 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO31

Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].

Alternate Names

DKFZp434B027, F-box only protein 31, F-box protein 31, FBX14DKFZp434J1815, Fbx31, FBXO14FLJ22477, MGC15419, MGC9527, pp2386, putative breast cancer tumor-suppressor, SCF ubiquitin ligase specificity factor

Entrez Gene IDs

79791 (Human)

Gene Symbol

FBXO31

Additional FBXO31 Products

Product Documents for FBXO31 Antibody (OTI1H1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXO31 Antibody (OTI1H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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