FBXO33 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91890

Novus Biologicals, part of Bio-Techne
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NBP1-91890
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FBXO33 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NNRNLQKFSLFGDISVLQQQGSLSNTYLSKVDPDGKKIKQIQQLFEEILSNSRQLKWLSCGFMLEIVTPTSLSSLSNAVANTMEHLSLLDNNIPGNSTLITAVELERFVNLHSLALDFCDFTAEMARVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXO33 Antibody

Western Blot: FBXO33 Antibody [NBP1-91890]

Western Blot: FBXO33 Antibody [NBP1-91890]

Western Blot: FBXO33 Antibody [NBP1-91890] - Analysis in control (vector only transfected HEK293T lysate) and FBXO33 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FBXO33 Antibody [NBP1-91890]

Immunocytochemistry/ Immunofluorescence: FBXO33 Antibody [NBP1-91890]

Immunocytochemistry/Immunofluorescence: FBXO33 Antibody [NBP1-91890] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FBXO33 Antibody [NBP1-91890]

Immunohistochemistry-Paraffin: FBXO33 Antibody [NBP1-91890]

Immunohistochemistry-Paraffin: FBXO33 Antibody [NBP1-91890] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Applications for FBXO33 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXO33

The FBXO33 gene encodes a 555 amino acid long, 62 kDA F-box only protein 33 that monitors the ubiquitination and proteasomal degradation of target proteins. It is thought to recognize as well as bind phosphorylated target proteins. FBXO33 is linked to osteoporosis and interacts with genes CUL1, IPO5, RBX1, HSPA9, and C1QBP.

Alternate Names

BMND12, c14_5247, F-box only protein 33, F-box protein 33, FBX33

Gene Symbol

FBXO33

Additional FBXO33 Products

Product Documents for FBXO33 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FBXO33 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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