FBXO4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52158

Novus Biologicals, part of Bio-Techne
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NBP1-52158
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FBXO4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EHTSAVNKMFSRH corresponding to internal region according to NP_277019.1.

Epitope

C-EHTSAVNKMFSRH

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Specificity

This antibody is expected to recognize isoform 1 and 2 (NP_036308.1; NP_277019.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FBXO4 Antibody

Immunohistochemistry-Paraffin: FBXO4 Antibody [NBP1-52158]

Immunohistochemistry-Paraffin: FBXO4 Antibody [NBP1-52158]

Immunohistochemistry-Paraffin: FBXO4 Antibody [NBP1-52158] - 2.5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for FBXO4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

detection limit 1:8000
Application Notes
Western blot: Preliminary experiments gave an approx 48kDa band in Human Heart ysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1kDa according to NP_036308.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Tonsil.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO4

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Alternate Names

DKFZp547N213, F-box protein 4, F-box protein Fbx4, FBX4F-box only protein 4, FLJ10141

Entrez Gene IDs

26272 (Human); 106052 (Mouse); 310363 (Rat)

Gene Symbol

FBXO4

UniProt

NP_036308.1 (Human)

Additional FBXO4 Products

Product Documents for FBXO4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FBXO4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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