Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

77.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO42 Antibody (OTI1H4)

Western Blot: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Western Blot: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO42.

Immunohistochemistry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Immunohistochemistry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of Human pancreas tissue.

Flow Cytometry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Flow Cytometry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by FBXO42 antibody.

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Applications for FBXO42 Antibody (OTI1H4)

Application

Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO42

F-box only protein 42 (FBXO42) is a substrate recognition component of the SKP1-CUL1-F-box protein (SCF) type E3 ubiquitin ligase complex. The FBXO42 protein recognizes p53/TP53; which promotes its ubiquitination and degradation. FBXO42 is a member of the F-box protein family. Members of this family are characterized by an approximately 40-amino acid F-box motif. F-box proteins are also known to interact with SKP1 and other ubiquitination targets through their interaction domains.

Alternate Names

F-box only protein 42, F-box protein 42, Fbx42, JFK, Just one F-box and Kelch domain-containing protein, KIAA1332FBX42

Gene Symbol

FBXO42

Additional FBXO42 Products

Product Documents for FBXO42 Antibody (OTI1H4)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for FBXO42 Antibody (OTI1H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

FBXO42 Antibody (OTI1H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45621

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all FBXO42 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for FBXO42 Antibody (OTI1H4)

Western Blot: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Immunohistochemistry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Flow Cytometry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Immunohistochemistry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Immunohistochemistry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Immunohistochemistry: FBXO42 Antibody (OTI1H4) [NBP2-45621]

Applications for FBXO42 Antibody (OTI1H4)

Flow Cytometry

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: FBXO42

Alternate Names

Gene Symbol

Additional FBXO42 Products

Product Documents for FBXO42 Antibody (OTI1H4)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for FBXO42 Antibody (OTI1H4)