Skip to main content

FBXO42 Antibody (OTI2G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03909

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-03909
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G7

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

77.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO42 Antibody (OTI2G7)

Western Blot: FBXO42 Antibody (2G7) [NBP2-03909] Analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO42 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: FBXO42 Antibody (OTI2G7) [NBP2-03909]

Immunohistochemistry-Paraffin: FBXO42 Antibody (OTI2G7) [NBP2-03909]

Immunohistochemistry-Paraffin: FBXO42 Antibody (2G7) [NBP2-03909] - Staining of paraffin-embedded Human colon tissue using anti-FBXO42 mouse monoclonal antibody.
Flow Cytometry: FBXO42 Antibody (OTI2G7) [NBP2-03909]

Flow Cytometry: FBXO42 Antibody (OTI2G7) [NBP2-03909]

Flow Cytometry: FBXO42 Antibody (2G7) [NBP2-03909] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO42 antibody, and then analyzed by flow cytometry.

Applications for FBXO42 Antibody (OTI2G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO42

Members of the F-box protein family, such as FBXO42, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (SKP1A; MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].

Alternate Names

F-box only protein 42, F-box protein 42, Fbx42, JFK, Just one F-box and Kelch domain-containing protein, KIAA1332FBX42

Entrez Gene IDs

54455 (Human)

Gene Symbol

FBXO42

Additional FBXO42 Products

Product Documents for FBXO42 Antibody (OTI2G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXO42 Antibody (OTI2G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...