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FBXO44 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84728

Novus Biologicals, part of Bio-Techne
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NBP1-84728
NBP1-84728-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARASGDLPARPGDHPAEERCQVEGGLPHILQLP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%). Reactivity reported in scientific literature (PMID: 23086937)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXO44 Antibody

Western Blot: FBXO44 Antibody [NBP1-84728]

Western Blot: FBXO44 Antibody [NBP1-84728]

Western Blot: FBXO44 Antibody [NBP1-84728] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-84728]

Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-84728]

Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-84728] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728]

Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728]

Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Applications for FBXO44 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25970626). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXO44

F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.

Alternate Names

FBG3 DKFZp781J0852, F-box gene 3, F-box only protein 44, F-box protein 44, F-box protein FBX30, F-box/G-domain protein 3, FBX30FBX6AFbx44, FBX44, Fbxo6a, MGC14140

Gene Symbol

FBXO44

Additional FBXO44 Products

Product Documents for FBXO44 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXO44 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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