Fc epsilon RI Antibody (1F2A9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37402

Novus Biologicals, part of Bio-Techne
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NBP2-37402
NBP2-37402-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F2A9

Format

BSA Free

Concentration

1 mg/ml

View all Fc epsilon RI Antibodies »

Product Summary for Fc epsilon RI Antibody (1F2A9) - BSA Free

Immunogen

Purified recombinant fragment of human Fc epsilon RI (AA: 42-103) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fc epsilon RI Antibody (1F2A9) - BSA Free

Western Blot: Fc epsilon RI Antibody (1F2A9)BSA Free [NBP2-37402]

Western Blot: Fc epsilon RI Antibody (1F2A9)BSA Free [NBP2-37402]

Western Blot: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Western blot analysis using FCER1A mouse mAb against SW620 (1), A549 (2), and A431 (3) cell lysate.
Flow Cytometry: Fc epsilon RI Antibody (1F2A9) - BSA Free [NBP2-37402]

Flow Cytometry: Fc epsilon RI Antibody (1F2A9) - BSA Free [NBP2-37402]

Flow Cytometry: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Flow cytometric analysis of HEK293 cells using FCER1A mouse mAb (green) and negative control (red).
Western Blot: Fc epsilon RI Antibody (1F2A9)BSA Free [NBP2-37402]

Western Blot: Fc epsilon RI Antibody (1F2A9)BSA Free [NBP2-37402]

Western Blot: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Western blot analysis using FCER1A mAb against human FCER1A recombinant protein. (Expected MW is 32.5 kDa)
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Applications for Fc epsilon RI Antibody (1F2A9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc epsilon RI

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of a a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Alternate Names

alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide

Gene Symbol

FCER1A

Additional Fc epsilon RI Products

Product Documents for Fc epsilon RI Antibody (1F2A9) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fc epsilon RI Antibody (1F2A9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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