Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # AER-37

Format

BSA Free

Concentration

0.5 mg/ml

View all Fc epsilon RI Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was FceRI alpha.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33741501).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Fc epsilon RI Antibody (AER-37) - BSA Free

Immunohistochemistry-Paraffin: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Immunohistochemistry-Paraffin: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Immunohistochemistry-Paraffin: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Staining of human mast cell in human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Flow Cytometry: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Flow Cytometry: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Flow Cytometry: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Analysis using the FITC conjugate of NBP1-43278. Staining of normal human peripheral blood cells with Anti-Human IgE PE and Mouse IgG2b K Isotype Control FITC (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) FITC (right).
Flow Cytometry: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Flow Cytometry: Fc epsilon RI Antibody (AER-37) - BSA Free [NBP1-43278]

Flow Cytometry: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

Applications for Fc epsilon RI Antibody (AER-37) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The AER-37 antibody has been tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fc epsilon RI

The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Alternate Names

alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide

Gene Symbol

FCER1A

Additional Fc epsilon RI Products

Product Documents for Fc epsilon RI Antibody (AER-37) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fc epsilon RI Antibody (AER-37) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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