Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20159
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Inhibition Activity
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # IV.3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated against the FcR of K562 cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free
Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] -
Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of IV.3 (NBP3-20159, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] -
Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of IV.3 (NBP3-20159) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20159, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Inhibition Activity
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Fc gamma RIIA/CD32a
Long Name
Fc gamma Receptor II A
Alternate Names
CD32a, FCGR2A, FcgRIIA, FCRIIA
Gene Symbol
FCGR2A
Additional Fc gamma RIIA/CD32a Products
Product Documents for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free
Product Specific Notices for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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