Fc gamma RIIA/CD32a Antibody (OTI9G5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47735

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9G5

Concentration

0.66 mg/ml

View all Fc gamma RIIA/CD32a Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

Specificity

This antibody is specific for Homo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32) (FCGR2A), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fc gamma RIIA/CD32a Antibody (OTI9G5)

Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Western Blot: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (OTI9G5) [NBP1-47735]

Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.
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Applications for Fc gamma RIIA/CD32a Antibody (OTI9G5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:200 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.66 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fc gamma RIIA/CD32a

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.Alternative splicing results in multiple transcript variants.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Entrez Gene IDs

2212 (Human)

Gene Symbol

FCGR2A

Additional Fc gamma RIIA/CD32a Products

Product Documents for Fc gamma RIIA/CD32a Antibody (OTI9G5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fc gamma RIIA/CD32a Antibody (OTI9G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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