FCF1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04075
![Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]](https://resources.bio-techne.com/images/products/FCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04075-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]")
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FCF1 Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-198 of human FCF1 (NP_057046.1). PCITDCVMAEIEKLGQKYRVALRIAKDPRFERLPCTHKGTYADDCLVQRVTQHKCYIVATVDRDLKRRIRKIPGVPIMYISNHRYNIERMPDDYGAPRF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FCF1 Antibody - Azide and BSA Free
![Western Blot: FCF1 AntibodyAzide and BSA Free [NBP3-04075]](https://resources.bio-techne.com/images/products/FCF1-Antibody-Western-Blot-NBP3-04075-img0003.jpg "Western Blot: FCF1 AntibodyAzide and BSA Free [NBP3-04075]")
Western Blot: FCF1 AntibodyAzide and BSA Free [NBP3-04075]
Western Blot: FCF1 Antibody [NBP3-04075] - Analysis of extracts of 293T cells, using FCF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit .![Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]](https://resources.bio-techne.com/images/products/FCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04075-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]")
Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]
Immunocytochemistry/Immunofluorescence: FCF1 Antibody [NBP3-04075] - Analysis of U-2 OS cells using FCF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: FCF1 Antibody - Azide and BSA Free [NBP3-04075]](https://resources.bio-techne.com/images/products/FCF1-Antibody-Immunohistochemistry-Paraffin-NBP3-04075-img0004.jpg "Immunohistochemistry-Paraffin: FCF1 Antibody - Azide and BSA Free [NBP3-04075]")
Immunohistochemistry-Paraffin: FCF1 Antibody - Azide and BSA Free [NBP3-04075]
Immunohistochemistry-Paraffin: FCF1 Antibody [NBP3-04075] - Immunohistochemistry of paraffin-embedded Human esophageal using FCF1 Rabbit pAb (NBP3-04075) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for FCF1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FCF1
Alternate Names
Bka, C14orf111, CGI-35, chromosome 14 open reading frame 111, FCF1 small subunit (SSU) processome component homolog (S. cerevisiae), MGC99629, rRNA-processing protein FCF1 homolog
Gene Symbol
FCF1
Additional FCF1 Products
Product Documents for FCF1 Antibody - Azide and BSA Free
Product Specific Notices for FCF1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov