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FcgR4/CD16-2 Antibody (011) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05889CL1

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunohistochemistry-Paraffin

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 011

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FcgR4/CD16-2 (Accession#: NP_653142.2; Met 1-Gln 203).

Reactivity Notes

Has cross-reactivity in ELISA with:

Human CD64

No cross-reactivity in ELISA with:

Human CD16a
Human CD16b
Human CD32a
Human CD32b
Mouse FCGR3

Specificity

Has cross-reactivity in ELISA with: Human CD64No cross-reactivity in ELISA with: Human CD16aHuman CD16bHuman CD32aHuman CD32bMouse FCGR3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for FcgR4/CD16-2 Antibody (011) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: FcgR4/CD16-2

Fcgr4, also known as Fc Receptor-like 3 (Fcrl3) and CD16-2, is a recently described mouse protein that is closely related to human Fc gamma RIIIA (60% amino acid identity) and may be its true ortholog. Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 mRNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.

Long Name

Fc receptor, IgG, low affinity IV

Alternate Names

CD16-2, Fcrl3

Gene Symbol

Fcgr4

Additional FcgR4/CD16-2 Products

Product Documents for FcgR4/CD16-2 Antibody (011) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FcgR4/CD16-2 Antibody (011) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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