FcgR4/CD16-2 Antibody (012)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05890

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-05890-100ul
NBP3-05890-50ul
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FcgR4/CD16-2 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FcgR4/CD16-2 (Accession#: NP_653142.2; Met 1-Gln 203).

Reactivity Notes

Has cross-reactivity in ELISA with:

Human CD64 / FCGR1A

No cross-reactivity in ELISA with:

Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3

Specificity

Has cross-reactivity in ELISA with: Human CD64 / FCGR1ANo cross-reactivity in ELISA with: Human CD16a / FCGR3A / FcRIIIa Human CD16b / FCGR3B / FcRIIIb Human CD32a / FCGR2A Human CD32b / FCGR2B Mouse CD16 / FCGR3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FcgR4/CD16-2 Antibody (012)

Immunocytochemistry/ Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890]

Immunocytochemistry/ Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890]

Immunocytochemistry/Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow Cytometry: FcgR4/CD16-2 Antibody (012) [NBP3-05890]

Flow Cytometry: FcgR4/CD16-2 Antibody (012) [NBP3-05890]

Flow Cytometry: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.

Applications for FcgR4/CD16-2 Antibody (012)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FcgR4/CD16-2

Fcgr4, also known as Fc Receptor-like 3 (Fcrl3) and CD16-2, is a recently described mouse protein that is closely related to human Fc gamma RIIIA (60% amino acid identity) and may be its true ortholog. Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 mRNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.

Long Name

Fc receptor, IgG, low affinity IV

Alternate Names

CD16-2, Fcrl3

Gene Symbol

Fcgr4

Additional FcgR4/CD16-2 Products

Product Documents for FcgR4/CD16-2 Antibody (012)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FcgR4/CD16-2 Antibody (012)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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