Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FCHO2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 to 175 of human FCH Domain Only 2 using the numbering given in entry NP_620137.2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FCHO2 Antibody

Western Blot: FCHO2 Antibody [NBP2-36524]

Western Blot: FCHO2 Antibody [NBP2-36524]

Western Blot: FCHO2 Antibody [NBP2-36524] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FCHO2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: FCHO2 Antibody [NBP2-36524]

Immunoprecipitation: FCHO2 Antibody [NBP2-36524]

Immunoprecipitation: FCHO2 Antibody [NBP2-36524] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated FCHO2, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for FCHO2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FCHO2

Alternate Names

FCH domain only 2

Gene Symbol

FCHO2

UniProt

NP_620137.2 (Human)

Additional FCHO2 Products

Product Documents for FCHO2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FCHO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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