FCHSD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12654
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human FCHSD1 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for FCHSD1 Antibody
![Western Blot: FCHSD1 Antibody [NBP3-12654]](https://resources.bio-techne.com/images/products/FCHSD1-Antibody-Western-Blot-NBP3-12654-img0001.jpg "Western Blot: FCHSD1 Antibody [NBP3-12654]")
Western Blot: FCHSD1 Antibody [NBP3-12654]
Western Blot: FCHSD1 Antibody [NBP3-12654] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.)![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody [NBP3-12654]](https://resources.bio-techne.com/images/products/FCHSD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12654-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody [NBP3-12654]")
Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody [NBP3-12654]
Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody [NBP3-12654] - Staining of FCHSD1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FCHSD1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.Applications for FCHSD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FCHSD1
Alternate Names
FCH and double SH3 domains 1, FCH and double SH3 domains protein 1, FLJ00007, nervous wreck homolog 2, NWK2
Gene Symbol
FCHSD1
Additional FCHSD1 Products
Product Specific Notices for FCHSD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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