FE65 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58360V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FE65 Antibodies »

Product Specifications

Immunogen

Recombinant rat FE65, expressed in bacteria. [Swiss-Prot# P46933]

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for FE65 Antibody [DyLight 405]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FE65

Fe65 (Protein Fe65 or APBB1) is a neuronal adaptor protein that mediates the assembly of multimolecular complexes through a variety of protein-protein interaction domains: the WW domain, which binds to proline-rich sequences, and two C-terminal phosphotyrosine-binding domains. FE65 is primarily found in CNS and is highly expressed in neurons of hippocampus, cerebellum, thalamus, and brain stem nuclei. Fe65 is localized within cytoplasm or nucleus, although its moderate amounts are tethered to membranes via binding with Alzheimer's disease amyloid precursor protein (APP). FE65 forms stable, transcriptionally active complex with AICD (APP intracellular domain) that follows nuclear translocation of AICD and apoptosis through Tip60. FE65 is functionally linked to cellular motility, morphology and actin dynamics through binding of its WW domain to actin-binding protein Mena. It plays a key role in response to DNA damage by translocating to nucleus and inducing apoptosis through recognizing/binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at DSBs, recruiting other pro-apoptosis factors such as MAPK8/JNK1. It is required for histone H4 acetylation DSBs and its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. It functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor that inhibits the expression of CASP4.

Alternate Names

adaptor protein FE65a2, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), amyloid beta A4 precursor protein-binding, family B, member 1, Fe65, FE65amyloid beta A4 precursor protein-binding family B member 1, MGC:9072, Protein Fe65, RIR, stat-like protein

Gene Symbol

APBB1

Additional FE65 Products

Product Documents for FE65 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FE65 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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