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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # C11D8

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Made to Viral FELINE LEUKAEMIA VIRUS gp70

Specificity

NB100-65565 recognizes the gp85/gp70 envelope glycoprotein of feline leukaemia virus. The antibody specifically recognizes the epitope defined by amino acids 214-218 of the gp70 sequence (aa MGPNL). Some weak cross-reactivity with the sequence MGPLL found in the FeLV p15e membrane protein may also occur. This antibody effectively neutralises all three FeLV subgroups (A,B and C).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Feline Leukaemia Virus gp70 Antibody (C11D8)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Removal of sodium azide is recommended prior to use in functional assays.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Feline Leukaemia Virus gp70

FeLV is a retrovirus (family Oncovirinae) that can cause severe immunosuppression, severe non regenerative anemia, and a variety of neoplasms. The 3 main subtypes (FeLVA, FeLVB and FeLVC) may act independently or together to cause a variety of diseases. The major envelope glycoprotein (gp70) is used to bind to target cells and varies with the FeLV subtype.

Alternate Names

Env polyprotein, Feline Leukemia Virus glycoprotein 70, Glycoprotein gp70 [Precursor], GP70 envelope protein, gp70 protein (SU), p15E protein, p15E protein (TM)

UniProt

Additional Feline Leukaemia Virus gp70 Products

Product Documents for Feline Leukaemia Virus gp70 Antibody (C11D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Feline Leukaemia Virus gp70 Antibody (C11D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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