Ferroportin/SLC40A1 Antibody (1308C) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75923
Recombinant Monoclonal Antibody.
![Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]](https://resources.bio-techne.com/images/products/Ferroportin-SLC40A1-Antibody-1308C-Flow-Cytometry-NBP2-75923-img0001.jpg "Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (94%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1308C
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Ferroportin/SLC40A1 Antibody (1308C) was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-320). [UniProt# Q9NP59]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ferroportin/SLC40A1 Antibody (1308C) - BSA Free
Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]
Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) [NBP2-75923] - HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (NBP2-75923) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).![Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]](https://resources.bio-techne.com/images/products/Ferroportin-SLC40A1-Antibody-1308C-Immunohistochemistry-Paraffin-NBP2-75923-img0002.jpg "Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]")
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - BSA Free [NBP2-75923]
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) [NBP2-75923] - Ferroportin/SLC40A1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (Catalog # NBP2-75923) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells.Applications for Ferroportin/SLC40A1 Antibody (1308C) - BSA Free
Application
Recommended Usage
Flow Cytometry
0.25 ug/ml
Immunohistochemistry
1 ug/ml
Immunohistochemistry-Paraffin
1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ferroportin/SLC40A1
FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).
References
1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657
2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604
3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207
4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869
Long Name
Solute Carrier Family 40 Member 1
Alternate Names
FPN1, HFE4, IREG1, MST079, MTP1, SLC11A3, SLC40A1
Gene Symbol
SLC40A1
Additional Ferroportin/SLC40A1 Products
Product Documents for Ferroportin/SLC40A1 Antibody (1308C) - BSA Free
Product Specific Notices for Ferroportin/SLC40A1 Antibody (1308C) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...