FEZ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53816

Novus Biologicals, part of Bio-Techne
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NB100-53816
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FEZ1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNKQKEQRELMKK corresponding to internal region according to NP_005094.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_005094.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FEZ1 Antibody

Western Blot: FEZ1 Antibody [NB100-53816]

Western Blot: FEZ1 Antibody [NB100-53816]

Western Blot: FEZ1 Antibody [NB100-53816] - Staining (0.1ug/ml) of Kelly cell lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816]

Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816]

Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Western Blot: FEZ1 Antibody [NB100-53816]

Western Blot: FEZ1 Antibody [NB100-53816]

Western Blot: FEZ1 Antibody [NB100-53816] - Staining (0.1ug/ml) of Rat Brain lysate (A) + Peptide (B) (35g protein in RIPA buffer). Detected by chemiluminescence.
View 1 more images

Applications for FEZ1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 8 ug/ml

Immunohistochemistry-Paraffin

5 - 8 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx 75 kDa band observed in Kelly and in Rat Brain lysates (calculated MW of 45.1 kDa according to NP_005094.1 and 45. 2kDa according to NP_112328.1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FEZ1

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Alternate Names

fasciculation and elongation protein zeta 1 (zygin I), fasciculation and elongation protein zeta-1, Zygin I, Zygin-1

Entrez Gene IDs

9638 (Human); 235180 (Mouse); 81730 (Rat)

Gene Symbol

FEZ1

UniProt

Q99689 (Human)

Additional FEZ1 Products

Product Documents for FEZ1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FEZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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