FGF basic/FGF2/bFGF Antibody (8W6M5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15367

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15367-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8W6M5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGF basic/FGF2/bFGF Antibodies »

Product Summary for FGF basic/FGF2/bFGF Antibody (8W6M5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FGF basic/FGF2/bFGF (NP_001997.5). RAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (8W6M5)

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Analysis of extracts of SKOV3 cells, using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Immunocytochemistry/Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Immunofluorescence analysis of HeLa cells using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
FGF basic/FGF2/bFGF Antibody (8W6M5)

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -Analysis of FGF2 in paraffin-embedded human lung squamous carcinoma tissue using FGF2 Rabbit mAb at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for FGF basic/FGF2/bFGF Antibody (8W6M5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (8W6M5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FGF basic/FGF2/bFGF Antibody (8W6M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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