I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: FGF basic/FGF2/bFGF Antibody (MC-GF1)
Brand: Novus Biologicals
SKU: NB600-778

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

FGF basic/FGF2/bFGF Antibody (MC-GF1)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-778

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # MC-GF1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Recombinant human bFGF

Localization

Secreted

Specificity

GF1 recognizes basic fibroblast growth factor (bFGF). The binds strongly to bFGF attached to proteoglycans, but is competitively displaced by heparin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for FGF basic/FGF2/bFGF Antibody (MC-GF1)

Application

Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Application Notes

Not suitable for detecting bFGF bound to cell surface receptors

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human)

Gene Symbol

FGF2

UniProt

P09038

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (MC-GF1)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (MC-GF1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

FGF basic/FGF2/bFGF Antibody (MC-GF1)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-778

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Clonality

Host

Isotype

Applications for FGF basic/FGF2/bFGF Antibody (MC-GF1)

ELISA

Immunohistochemistry

Immunohistochemistry-Frozen

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: FGF basic/FGF2/bFGF

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (MC-GF1)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (MC-GF1)