FGFR1 Antibody (M17D10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80733

Novus Biologicals, part of Bio-Techne
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NBP2-80733
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BSA Free
NB100-2080
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M17D10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all FGFR1 Antibodies »

Product Summary for FGFR1 Antibody (M17D10) - Azide and BSA Free

Immunogen

Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

Epitope is within the sequence pro179 to lys195 in Ig loop II.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 8575296). Not yet tested in other species.

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FGFR1 Antibody (M17D10) - Azide and BSA Free

Western Blot: FGFR1 Antibody (M17D10)Azide and BSA Free [NBP2-80733]

Western Blot: FGFR1 Antibody (M17D10)Azide and BSA Free [NBP2-80733]

Western Blot: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody. Image from the standard format of this antibody.
Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein. Image from the standard forma
Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 prot
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Applications for FGFR1 Antibody (M17D10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M17D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FGFR1 Antibody (M17D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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