FGFR5/FGFRL1 Antibody (019)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90467

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 019

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGFR5/FGFRL1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FGFR5/FGFRL1 (Accession#: NP_473412.1; Met1-Pro374).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FGFR5/FGFRL1 Antibody (019)

Western Blot: FGFR5/FGFRL1 Antibody (019) [NBP2-90467]

Western Blot: FGFR5/FGFRL1 Antibody (019) [NBP2-90467]

Western Blot: FGFR5/FGFRL1 Antibody (019) [NBP2-90467] - Anti-FGFR5/FGFRL1 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 62 kDa
Immunoprecipitation: FGFR5/FGFRL1 Antibody (019) [NBP2-90467]

Immunoprecipitation: FGFR5/FGFRL1 Antibody (019) [NBP2-90467]

Immunoprecipitation: FGFR5/FGFRL1 Antibody (019) [NBP2-90467] - Mouse FGFR5/FGFRL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-Mouse FGFR5/FGFRL1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse FGFR5/FGFRL1 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 70 kDa

Applications for FGFR5/FGFRL1 Antibody (019)

Application
Recommended Usage

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR5/FGFRL1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor-like 1

Alternate Names

FGF R5, FGFRL1

Gene Symbol

FGFRL1

Additional FGFR5/FGFRL1 Products

Product Documents for FGFR5/FGFRL1 Antibody (019)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FGFR5/FGFRL1 Antibody (019)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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