Fgr Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85677

Novus Biologicals, part of Bio-Techne
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NBP1-85677
NBP1-85677-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fgr Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEARTEDDLTFTKGEKFHILNNTEGDWWEARSLSSGKTGCIPSNYVAPVDSIQAEEWYFGKIG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fgr Antibody

Western Blot: Fgr Antibody [NBP1-85677]

Western Blot: Fgr Antibody [NBP1-85677]

Western Blot: Fgr Antibody [NBP1-85677] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431
Immunocytochemistry/ Immunofluorescence: Fgr Antibody [NBP1-85677]

Immunocytochemistry/ Immunofluorescence: Fgr Antibody [NBP1-85677]

Immunocytochemistry/Immunofluorescence: Fgr Antibody [NBP1-85677] - Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane & aggresome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677]

Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677]

Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human Lymph node shows moderate cytoplasmic and membranous positivity in germinal center and non-germinal center cells.
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Applications for Fgr Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fgr

Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.

Long Name

Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog

Alternate Names

Src2

Gene Symbol

FGR

Additional Fgr Products

Product Documents for Fgr Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fgr Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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