FHL1 Antibody (OTI3G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02654

Novus Biologicals, part of Bio-Techne
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NBP2-02654
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FHL1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FHL1 Antibody (OTI3G7)

Western Blot: FHL1 Antibody (3G7) [NBP2-02654] Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) [NBP2-02654]

Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) [NBP2-02654]

Immunocytochemistry/Immunofluorescence: FHL1 Antibody (3G7) [NBP2-02654] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.
Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]

Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]

Flow Cytometry: FHL1 Antibody (3G7) [NBP2-02654] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for FHL1 Antibody (OTI3G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FHL1

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

Long Name

Four and a Half LIM Domains Protein 1

Alternate Names

HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA

Entrez Gene IDs

2273 (Human)

Gene Symbol

FHL1

Additional FHL1 Products

Product Documents for FHL1 Antibody (OTI3G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FHL1 Antibody (OTI3G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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