FHL1 Antibody (OTI3G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02654
![Western Blot: FHL1 Antibody (OTI3G7) [NBP2-02654]](https://resources.bio-techne.com/images/products/FHL1-Antibody-3G7-Western-Blot-NBP2-02654-img0003.jpg "Western Blot: FHL1 Antibody (OTI3G7) [NBP2-02654]")
![Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]](https://resources.bio-techne.com/images/products/FHL1-Antibody-3G7-Flow-Cytometry-NBP2-02654-img0001.jpg "Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3G7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FHL1 Antibody (OTI3G7)
Immunogen
Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FHL1 Antibody (OTI3G7)
Western Blot: FHL1 Antibody (3G7) [NBP2-02654] Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) [NBP2-02654]](https://resources.bio-techne.com/images/products/FHL1-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP2-02654-img0004.jpg "Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) [NBP2-02654]")
Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) [NBP2-02654]
Immunocytochemistry/Immunofluorescence: FHL1 Antibody (3G7) [NBP2-02654] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.![Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]](https://resources.bio-techne.com/images/products/FHL1-Antibody-3G7-Flow-Cytometry-NBP2-02654-img0002.jpg "Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]")
Flow Cytometry: FHL1 Antibody (OTI3G7) [NBP2-02654]
Flow Cytometry: FHL1 Antibody (3G7) [NBP2-02654] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for FHL1 Antibody (OTI3G7)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FHL1
Long Name
Four and a Half LIM Domains Protein 1
Alternate Names
HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA
Entrez Gene IDs
2273 (Human)
Gene Symbol
FHL1
Additional FHL1 Products
Product Documents for FHL1 Antibody (OTI3G7)
Product Specific Notices for FHL1 Antibody (OTI3G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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