FHL1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1461
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CTVSRVSHPVSKARK corresponding to internal region according to AAC72886.1.
Specificity
This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for FHL1 Antibody
![Western Blot: FHL1 Antibody [NB100-1461]](https://resources.bio-techne.com/images/products/FHL1-Antibody-Western-Blot-NB100-1461-img0001.jpg "Western Blot: FHL1 Antibody [NB100-1461]")
Western Blot: FHL1 Antibody [NB100-1461]
Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Western Blot: FHL1 Antibody [NB100-1461]](https://resources.bio-techne.com/images/products/FHL1-Antibody-Western-Blot-NB100-1461-img0002.jpg "Western Blot: FHL1 Antibody [NB100-1461]")
Western Blot: FHL1 Antibody [NB100-1461]
Western Blot: FHL1 Antibody [NB100-1461] - Proteins interacting with HACE1 accumulate in Hace1-/-sTAC heart. Representative western blots show increased accumulation of some of the HACE1-interacting proteins in the Hace1-/-sTAC myocardium. n=3 mice per group, error bars represent s.e.m., P-value between KO and WT sTAC as indicated (one-way analysis of variance). GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms4430), licensed under a CC-BY license.Applications for FHL1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:128000
Western Blot
1:100-1:2000
Application Notes
WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 36.3 kDa band according to AAC72886) is consistent with scientific literature (PMID: 1048092).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FHL1
Long Name
Four and a Half LIM Domains Protein 1
Alternate Names
HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA
Entrez Gene IDs
2273 (Human)
Gene Symbol
FHL1
UniProt
Additional FHL1 Products
Product Documents for FHL1 Antibody
Product Specific Notices for FHL1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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