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FHL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-804

Novus Biologicals, part of Bio-Techne
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NB300-804

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence RDDILCPDCGKDI corresponding to C-Terminus according to NP_001441.4.

Specificity

Reported variants represent identical protein (NP_001441.4; NP_963849.1; NP_963851.2; NP_001034581.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FHL2 Antibody

Western Blot: FHL2 Antibody [NB300-804]

Western Blot: FHL2 Antibody [NB300-804]

Western Blot: FHL2 Antibody [NB300-804] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for FHL2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human and mouse heart lysates (calculated MW of 32.2 kDa band according to NP_001441.4).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FHL2

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain. FHL2 might enforce beta-catenin transactivation activity in cancer cells.

Long Name

Four and a Half LIM Domains Protein 2

Alternate Names

AAG11, DRAL, SLIM3

Entrez Gene IDs

2274 (Human); 14200 (Mouse); 63839 (Rat)

Gene Symbol

FHL2

Additional FHL2 Products

Product Documents for FHL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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