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FHL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33603

Novus Biologicals, part of Bio-Techne
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NBP1-33603

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for FHL2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human FHL2. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FHL2 Antibody

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-33603]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-33603]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-33603] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.
FHL2 Antibody

Western Blot: FHL2 Antibody [NBP1-33603] -

Western Blot: FHL2 Antibody [NBP1-33603] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL2 antibody (NBP1-33603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FHL2 Antibody

Western Blot: FHL2 Antibody [NBP1-33603] -

Western Blot: FHL2 Antibody [NBP1-33603] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL2 antibody (NBP1-33603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FHL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FHL2

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Long Name

Four and a Half LIM Domains Protein 2

Alternate Names

AAG11, DRAL, SLIM3

Entrez Gene IDs

2274 (Human); 14200 (Mouse)

Gene Symbol

FHL2

UniProt

Additional FHL2 Products

Product Documents for FHL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.